Condition of a patient model development for ulceration mechanism elucidation following pressure loading in protein malnourished rats

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659956
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Fundamental nursing
• Research Institution: Ishikawa Prefectural Nursing University
• Principal Investigator: KITAYAMA Yukie 石川県立看護大学, 看護学部, 准教授 (20419740)
• Co-Investigator(Kenkyū-buntansha): SUGAMA Junko 金沢大学, 保健学系, 教授 (00203307) ; NAKATANI Toshio 金沢大学, 保健学系, 教授 (60198124)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 低蛋白血症 / ラット / 褥瘡発生 / 創傷モデル / HMGB1 / ネクローシス / CC3a / アポトーシス

Outline of Final Research Achievements

There was a nourishment drop in a cause of the pressure ulcer development that was the issue of elderly care in Japan. But there was no study that determined the skin tissue changes. We observed views at pressure ulcer development using protein malnourished rats (0% group). ※ We obtained approval of Kanazawa University animal experiment Committee.
With the normal skin (HE staining), there was no great difference in the ratio of epidermal thickness, cutaneous muscle and subcutaneous fatty layer and the both groups. Because the ratio of skeletal muscle decreased in the 0% group, it was suggested by load in the malnutrition that the tissue damage in the deep part.
The apoptotic (CC3a staining) views were not seen in p in which during 24 hours from the pressure cancelled time. However, is the HMGB1 staining indicating necrotic views; a pressure side border part (Ep) caught the damage most.

Free Research Field

創傷看護