Establishment of novel technique using primordial germ cells induced from embryonic stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24680045
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Kyoto University
• Principal Investigator: OHTA Hiroshi 京都大学, 医学(系)研究科(研究院), 助教 (50391892)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 始原生殖細胞 / ES細胞 / 小分子化合物 / 増殖・分化 / 精子形成

Outline of Final Research Achievements

We have recently succeeded in establishing the culture system to induce primordial germ cell like cells (PGCLCs), which possible to differentiate functional sperm or oocytes, from ES cells or iPS cells. In this study, we performed chemical library screening using PGCLCs and identified the chemicals possible to support the proliferation of PGCLCs for a certain period in vitro. This proliferated PGCLC population in vitro was able to differentiate functional sperm when the cells were trasnplanted into the tetes of infertile male mice. Further improvement of this culture system will allowed us to regulate the proliferation and differentiation of PGCLCs in vitro.

Free Research Field

発生生物学