2013 Fiscal Year Final Research Report
Understanding molecular mechanisms in human ES/iPS cell renewal and development of defined culture system
| Project/Area Number |
24680052
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto University |
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Principal Investigator |
HASEGAWA Kouichi 京都大学, 物質-細胞統合システム拠点, 講師 (50378890)
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| Research Collaborator |
KAHN Michael University of Southern California, 教授
WELLS Christen The University of Queensland, 准教授
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 再生医工学材料 / 化学合成培地 |
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| Research Abstract |
The extrinsic factors regulating human ES/iPS cell self-renewal are incompletely understood to date, but bFGF and TGFß signaling form the cornerstone of most systems for human ES/iPS cell propagation. In previous study, we have identified a DYRK inhibitor that could modulate Wnt pathway and support Wnt-induced self-renewal without inducing differentiation. Utilizing Wnt and the compound, we have developed a defined xeno-free culture system that allows for long-term expansion of human ES/iPS cells without FGF/TGFß supplementation. In further investigation, we have identified several key pathways involved in DYRK inhibition/Wnt-dependent self-renewal and found small chemical compounds can control these. Utilizing these chemical compounds, we have developed a novel and simple defined-culture system for human ES/iPS cells.
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[Journal Article] A Novel 3D Sphere Culture System Containing Functional Polymers for Large-scale Human Pluripotent Stem Cell Production2014
Author(s)
Tomomi G. Otsuji, Jiang Bin, Azumi Yoshimura, Misayo Tomura, Daiki Tateyama, Itsunari Minami, Yoshihiro Yoshikawa, Kazuhiro Aiba, John E. Heuser, Taito Nishino, Kouichi Hasegawa and Norio Nakatsuji
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Journal Title
Stem Cell Report
Volume: 2(5)
Pages: 734-745
Peer Reviewed
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[Journal Article] Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells2014
Author(s)
Li Liu, Momoko Yoshioka, Minako Nakajima, Arata Ogasawara, Jun Liu, Kouichi Hasegawa, Sisi Li, Jianli Zou, Norio Nakatsuji, Ken-ichiro Kamei, Yong Chen
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Journal Title
Biomaterials
Volume: 35(24)
Pages: 6259-6267
Peer Reviewed
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