Identification of long non-coding RNAs critically involved in colon cancer

2014 Fiscal Year Final Research Report

• Project/Area Number: 24680092
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Tumor diagnosis
• Research Institution: Sapporo Medical University
• Principal Investigator: MARUYAMA Reo 札幌医科大学, 医学部, 准教授 (60607985)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: lncRNA

Outline of Final Research Achievements

There is growing evidence that long non-coding RNAs (lncRNAs) play important roles in a wide repertoire of biological processes. It is very likely that lncRNAs also play critical roles in colorectal cancer (CRC). However, few studies have reported the involvement of lncRNAs in CRC. In this study, we aimed to comprehensively identify novel lncRNAs involved in CRC. To search for novel lncRNAs, we used ChIP-seq technology to obtain histone methylation profiles in endoscopic biopsy specimens. We characterized global histone modification plofiles in more than 20 normal tissues and cancers. We then examined histone status around putative lncRNAs and generated a list of candidate lncRNAs. In order to narrow the list down, we also utilized publicly available DNA methylation data as well as RNASeq to perform integrated analysis. Finally we identified ~10 lncRNAs silenced by DNA methylation and also identified ~10 lncRNAs that are abnormally overexpressed in CRC.

Free Research Field

分子生物学