High-sensitive proteomic analysis of proteins modified in response to DNA damage.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24681009
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: ADACHI Jun 独立行政法人医薬基盤研究所, その他部局等, 研究員 (20437255)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: DNA損傷応答 / シグナル伝達 / 翻訳後修飾

Outline of Final Research Achievements

In order to gain broader insight into the phosphorylation signal controlling DNA damage response (DDR), system-wide analysis of phosphorylation dynamics after gamma irradiation has been investigated. 25971 phosphosites were identified with accurate quantification. 6555 phosphosites displayed dynamic changes in phosphorylation status during one hour after irradiation. We identified distinct dynamic phosphorylation patterns of kinases, which had not been known to be associated with DNA damage, by the bioinformatics analysis based on matching sequence motif and protein-protein interaction. We found that DNA damage signaling is activated and colony formation is inhibited by the inhibition of the kinases.

Free Research Field

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