2014 Fiscal Year Final Research Report
A single mRNA counting by a microchamber array device containing a single human nucleus
| Project/Area Number |
24681027
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Nagoya University |
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Principal Investigator |
KAJI Noritada 名古屋大学, 工学(系)研究科(研究院), 准教授 (90402479)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ナノバイオ / mRNA / 細胞 |
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| Outline of Final Research Achievements |
In this research, a microchamber array device was designed to capture a single cell, lyse a cell membrane, and isolate a single cell nucleus, and then mRNA in a single cell nucleus were directly quantitated by using highly sensitive In-Stem Molecular Beacons (ISMB). As a result, the expression of three frequently used house-keeping genes were successfully quantitated to an accuracy of 100 molecules within a single cell nucleus.
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| Free Research Field |
ナノバイオテクノロジー
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