Dissection of epigenome dynamics of germ cel specification pathway through establishement of a ChIP-seq method from small number of cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24681039
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Genome biology
• Research Institution: Kyoto University
• Principal Investigator: KURIMOTO Kazuki 京都大学, 医学(系)研究科(研究院), 助教 (20415152)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ChIP-seq / BLIMP1 / primordial germ cell / 始原生殖細胞 / 微量 / 次世代シークエンサー / 生殖細胞 / エピゲノム

Outline of Final Research Achievements

The identity of all of the cells that comprise induviduals are defined by the cellular epigenom in the nuclei. Due to technical limitation,genome-wide analysis of binding sites of transcriptional regulators are almost limited to cell types for which many cells are abvailable, including hematopoietic cells and cultured cells. I established a method for ChIP-seq of transcriptiopn factors from small number of cells and enabled identification of the genome-wide binding sites of transcription factor at 1/100 ~ 1/1000 time previous scale. Using thise method, Using this method, I determined binding sites of two key transcription factors for germ cell specificatin pathway, BLIMP1 and T (Brachyury), in vitro. In addition, I determined representative histone modifications associated to active transcription and repression, and revealed landscape of chromatin dynamics during the PGC specifciation in vitro.

Free Research Field

分子生物学・ゲノム科学