2015 Fiscal Year Final Research Report
Development of fluorescent photoswitchable molecules toward RESOLFT super-resolution fluorescence microscope
| Project/Area Number |
24685021
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Functional materials chemistry
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| Research Institution | Kumamoto University (2014-2015)
Hokkaido University (2012-2013) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 蛍光 / フォトクロミズム / 超解像蛍光イメージング / ジアリールエテン |
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| Outline of Final Research Achievements |
We tried to develop the suitable fluorescent photoswitchable molecules, which have photoreactivity for visible-light and show the stable fluorescence photoswitching even in the aqueous conditions, toward RESOLFT super-resolution fluorescence microscope in this study.
In this study, we successfully prepared a visible-light reactive diarylethene (DAE) derivative by introducing an aromatic dye at the reactive carbon atom of the DAE unit, optimizing orbital level of each component, and controlling the mutual orientation of the aromatic dye and the DAE unit.
In addition, we successfully prepared a highly efficient fluorescent photoswitchable nanoparticle (NP). Such NPs show the non-linear fluorescence photoswitching behavior at very low photoreaction conversion yield. This “giant amplification of fluorescence photoswitching” represent an extremely promising and challenging step toward the application for RESOLFT super-resolution fluorescence microscope.
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| Free Research Field |
光化学
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