Development of highly sensitive in vivo imaging technology based on advanced functional nanoparticle design

2015 Fiscal Year Final Research Report

• Project/Area Number: 24685028
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Osaka University
• Principal Investigator: Mizukami Shin 大阪大学, 工学(系)研究科(研究院), 准教授 (30420433)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 可視化プローブ / MRI / コア－シェルナノ粒子

Outline of Final Research Achievements

Functional 19F MRI probes were developed in order to visualize biological functions in deep regions of living animals. First, to improve the sensitivity of conventional small-molecule 19F MRI probes, new nanoparticle-based material was developed. This nanoparticle consists of perfluorocarbon core and silica shell. Then, the attenuation method of the 19F MRI signal of this nanomateria was developed by modifying the surface with lots of Gd3+ complexes. By introducing hydrolase substrate with the linker between a Gd3+ complex and the silica surface, highly sensitive nanoparticle-based 19F MRI probes to detect enzyme activities were developed. By using one of the probes, enzyme activities in a living animal was visualized by 19F MRI.

Free Research Field

ケミカルバイオロジー