Optical Manipulation and Measurment Using Optically-Controlled Gel-Tool for intracellular Analysis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24686032
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Nagoya University
• Principal Investigator: HISATAKA Maruyama 名古屋大学, 工学(系)研究科(研究院), 准教授 (60377843)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: マイクロ・ナノメカトロニクス / 光ピンセット / フォトクロミック材料 / マイクロ流体チップ

Outline of Final Research Achievements

We developed selective and rapid injection of fluorescence sensor encapsulated in the artificial biomembrane into the cell for intracellular measurement. The biomembrane contains photochromic chemical, 1, 3, 3-Trimethylindolino-6'- nitrobenzopyrylospiran (SP), for optical control of zeta potential. The structure of SP changes between spiropyran (SP) type and merocyanine (MC) type by UV/VIS illumination. Zeta potential of the biomembrne is switched from negative to positive by photoisomerization of SP. The sensor in the positive-charged biomembrane adheres to the cell membrane by electrostatic force since the cell membrane is negative-charged. The cyclic mechanical stimulus using optical tweezers is applied to the cell membrane for rapid injection of the sensor by membrane fusion and endocytosis. We demonstrated selective adhesion of the liposome to the cell membrane by optical control of zeta potential. We also demonstrated short-time injection of 1 um fluorescence sensor.

Free Research Field

マイクロ・ナノメカトロニクス