2015 Fiscal Year Final Research Report
The molecular basis of the functional retinal circuitry
| Project/Area Number |
24687010
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Onishi Akishi 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 研究員 (70569102)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 網膜 / 視細胞 / 中心窩 |
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| Outline of Final Research Achievements |
In this study, we performed functional screening of molecules that govern specific synaptic connection between photoreceptors (rod, S cone and M cone) and secondary neurons (horizontal cell and bipolar cells) in the mouse retina. When we electroporated Susd3 cDNA, a single transmembrane protein specifically expressed in rod photoreceptors in the mouse retinas, the photoreceptors showed branching synaptic terminals that targeted secondary neurons in a dose dependent manner. Next, we developed double knockout mice of Aldh1a1 and Cyp1b1, enzymes that synthesize ligands for Rxrg, and observed significant decrease of M cones. In addition, Aldh1a1 deficient mice showed choroidal hypoplasia.
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| Free Research Field |
分子発生学
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