2013 Fiscal Year Final Research Report
Structural basis of membrane protein insertion
| Project/Area Number |
24687016
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Nara Institute of Science and Technology (2013)
The University of Tokyo (2012) |
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Principal Investigator |
TSUKAZAKI Tomoya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80436716)
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| Research Collaborator |
濡木 理 東京大学, 大学院理学系研究科, 教授 (10272460)
熊崎 薫 東京大学, 大学院理学系研究科, 大学院生
千葉 志信 京都産業大学, 総合生命科学部, 准教授 (20523517)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | タンパク質 / トランスロコン / 膜組み込み / 膜透過 / YidC / Sec / 膜タンパク質 / 構造生物学 |
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| Research Abstract |
Protein integration is an essential process in all living cells. In this study, we determined the crystal structure of YidC, an membrane protein insertase, at 2.4 A resolution and showed by structural-based functional analyses that YidC provides a hydrophilic cavity for membrane protein insertion. Finally, we proposed a new model of the molecular mechanism of YidC-mediated protein insertion into the cytoplasmic membrane.
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