Analysis of coordination mechanism of multiple bio-molecules using DNA-origami base molecular nano-layout technology.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24687018
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biophysics
• Research Institution: Kyoto University (2014); The University of Tokyo (2012-2013)
• Principal Investigator: TADAKUMA Hisashi 京都大学, 物質-細胞統合システム拠点, 研究員 (10339707)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: １分子計測・操作 / ナノマシン / 蛋白質 / 核酸

Outline of Final Research Achievements

In the cell, many factors orchestrate complicated but elegant system. However, how multiple biomolecules are coordinated each other remains elusive. Here we created gene nano-chip using DNA-Origami-tile as the skeletal structure and RNA polymerase (RNAP) as the functional module, allowing us a rational design of orthogonal gene expression device. Our approach of integrating biomolecules on DNA-origami-tile base nano-chip might contribute to explore the precise mechanism of fundamental phenomena and also to develop nano-devices.

Free Research Field

生物物理学