High-throughput analysis of single-cell gene expression in higher organisms

2015 Fiscal Year Final Research Report

• Project/Area Number: 24687022
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biophysics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Taniguchi Yuichi 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (90556276)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: １分子・１細胞生物学 / 生物物理学 / システム生物学 / 超高感度顕微鏡技術 / 微細加工技術 / 生命反応の物理

Outline of Final Research Achievements

We have carried out developments for technological tools to investigate single-molecule gene expression dynamics in single cells of higher organisms in a high-throughput manner. To realize this, we have developed a light-sheet illumination fluorescence microscopy that enables 3D volumetric imaging within the whole volume of thick cells set on a multi-well plate. In addition, we have developed a probing technique to detect mRNA and protein expressions at single molecule levels. Through analysis of gene expression dynamics in Saccharomyces cerevisiae cells for multiple gene targets, we have found a common feature in mechanisms on gene expression noise.

Free Research Field

生物学