2015 Fiscal Year Final Research Report
Unveiling the mode of unreduced pollen formation in 'Nishiuchi Konatsu' Hyuganatsu and its application for citrus breeding
| Project/Area Number |
24688003
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 園芸学 / 果樹 / カンキツ / 倍数性 / 非還元花粉 |
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| Outline of Final Research Achievements |
This research object is to gain the knowledge about the mode of unreduced pollen formation of ‘Nishiuchi Konatsu’ Hyuganatsu and to improve the recovery rate of plantlet by the embryo rescue. Six heterozygotic SSR markers, which are located on the same chromosome in ‘Nishiuchi Konatsu’, were screened. Unreduced pollen grains were genotyped for these SSR loci by the single-pollen genotyping. The inheritance of heterozygosity implied the mode of unreduced pollen formation was occurred through first division restitution (FDR). For embryo rescue, it was revealed that gibberellic acid was effective only for the initial culture and the fruit of 40-50 mm in diameter were appropriate for the embryo rescue.
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| Free Research Field |
果樹園芸学
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