The functional analysis of kidney stone-phagocytosis activity by the macrophages and the application to dissolution therapy.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24689060
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: OKADA Atsushi 名古屋市立大学, 医学(系)研究科(研究院), 講師 (70444966)
• Research Collaborator: KOHRI Kenjiro 名古屋市立大学, 大学院医学研究科, 学長 (30122047) ; TOZAWA Keiichi 名古屋市立大学, 大学院医学研究科, 准教授 (40264733) ; YASUI Takahiro 名古屋市立大学, 大学院医学研究科, 教授 (40326153) ; HAMAMOTO Shuzo 名古屋市立大学, 大学院医学研究科, 助教 (80551267) ; HIROSE Masahito 名古屋市立大学, 大学院医学研究科, 研究員 (70529172) ; NIIMI Kazuhiro 名古屋市立大学, 大学院医学研究科, 研究員 (70551274) ; TAGUCHI Kazumi 名古屋市立大学, 大学院医学研究科, 研究員 (00595184) ; FUJII Yasuhiro 名古屋市立大学, 大学院医学研究科, 研究員 (30566229)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 尿路結石 / マクロファージ / op/opマウス / マルチプレックス解析 / 結晶貪食モデル

Outline of Final Research Achievements

This study is aimed establish a technique to control the function of macrophages as a new prevention factor for kidney stone formation by positioning that the main constituent of the “stone elimination” phenomenon is "the kidney stone-phagocytosis activity" of Mφ, and we performed three following studies using an animal model, human specimen and culture model.
[1] We identified stone-preventive ability of M-CSF-derived M2-Mφ by increased stone formation in Mφ-deficient mice (op/op). [2] We showed the possibility that lower urinary IL-4 related in stone formation using multiplex analysis. [3] Using culture Mφ, we identified that the englobed COM crystals were revealed in lysosomes of Mφ with acidified environment and disappeared over time.

Free Research Field

医歯薬学