• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2014 Fiscal Year Final Research Report

Membrane dynamics and fatty acid elongation in the growth cone

Research Project

  • PDF
Project/Area Number 24700318
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neuroscience in general
Research InstitutionNiigata University

Principal Investigator

NOZUMI MOTOHIRO  新潟大学, 医歯学系, 講師 (00420323)

Project Period (FY) 2012-04-01 – 2015-03-31
Keywords神経科学
Outline of Final Research Achievements

The growth cone is a highly motile structure in the growing tip of a neurite at the stages of the neuronal development and regeneration. To visualize the vesicular trafficking and actin reorganization of the growth cone in detail simultaneously, we analyzed the dynamics of GFP-synaptophysin and mCherry-actin in the growth cone of NG108-15 cells, using a superresolution microscopy. We found that the vesicles arose near the root portions of filopodia, and most of them were retrogradely moving along the actin bundles. Both of such events were inhibited by actin polymerization inhibitors or a myosin light-chain kinase inhibitor. RNAi of fascin also decreased the numbers of vesicles localized at the leading edge. Moreover, the retrogradely moving vesicles were not colocalized with clathrin but with endophilin and dynamin. These results suggest that there is a novel mechanism of membrane retrieval in the growth cone.

Free Research Field

細胞生物学

URL: 

Published: 2016-06-03  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi