Search for the marker of chandelier cells in cerebral cortex

2013 Fiscal Year Final Research Report

• Project/Area Number: 24700327
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: Kumamoto University
• Principal Investigator: ESUMI Shigeyuki 熊本大学, 生命科学研究部, 助教 (90404334)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 大脳皮質GABAニューロン / シャンデリア細胞 / マイクロアレイ / 大脳皮質 / 抑制性神経細胞 / 細胞系譜

Research Abstract

The chandelier cells are the most distinct GABAergic neurons that innervate the axon initial segment of pyramidal neurons. In spite of particular morphology and function, there is no chandelier neuron markers were found. Recently, it has been reported that the chandelier cell is enriched in transplants from the vMGE at embryonic day 15 (Inan et al., 2012). In addition, a number of cortical chandelier cells were labeled in Nkx2-1CreER mouse (Taniguchi et al., 2013). To search chandelier neuron marker, we transplanted the E15.5 vMGE cells that derived from BAC- PV- Cre transgenic mouse to P0 mouse cortex. Nonetheless, there are no chandelier cells were found in the transplanted adult mouse cortex. Next, we injected tamoxifen to E17.5 Nkx2-1CreER; R26-YFP mouse for labeling chandelier cells. As the results, GFP positive progenitor cells were found in VZ and SVZ in the P0 mouse. In future, we plan to pick up GFP positive progenitor and try single-cell microarray analysis.

Research Products

• [Journal Article] Amygdala kindling induces nestin expression in the leptomeninges of the neocortex (2013)
  • Author(s): Ninomiya S, Esumi S, Ohta K, Fukuda T, Ito T, Imayoshi I, Kageyama R, Ikeda T, Itohara S, Tamamaki N.
  • Journal Title: Neuroscience Research Volume: 75(2) Pages: 121-129
  • Peer Reviewed
• [Presentation] 内側扁桃体におけるDbx1由来神経細胞の解析 (2014)
  • Author(s): 江角重行,Yasmin Kamal,Katie Sokolowski,平田務,Peijun Li,Alessandra Pierani,,Molly Huntsman,Nirao Shah,玉巻伸章and Joshua G.Corbin
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 栃木県下野市薬師寺自治医科大学キャンパス
  • Year and Date: 20140327-29
• [Presentation] 内側扁桃体におけるDbx1由来神経細胞の解析 (2014)
  • Author(s): 江角重行,Yasmin Kamal,Katie Sokolowski,平田務,Peijun Li,Alessandra Pierani,,Molly Huntsman,Nirao Shah,玉巻伸章 and Joshua G.Corbin
  • Organizer: 第7回神経発生討論会
  • Place of Presentation: 大阪府吹田市大阪大学吹田キャンパス銀杏会館
  • Year and Date: 20140313-14
• [Presentation] Generation of sexually dimorphic limbic system neuronal populations from Dbxl+ embryonic progenitor pools (2013)
  • Author(s): Shigeyuki Esumi, Yasmin Kamal, Katie Sokolowskil, Tsutomu Hirata, Peijun Li, Alessandra Pierani, Nobuaki Tamamaki, Molly Huntsman, Nirao Shah and Joshua G. Corbin
  • Organizer: Gordon Research Conference (Amygdala and Health)
  • Place of Presentation: Stonehill college, Boston USA)
  • Year and Date: 20130728-0802
• [Presentation] 内側扁桃体におけるDbx1由来神経細胞の解析 (2013)
  • Author(s): 江角重行
  • Organizer: 第19回日本行動神経内分泌学研究会全国集会
  • Place of Presentation: 鹿児島県桜島横山町レインボー桜島
  • Year and Date: 20130705-06
• [Presentation] 内側扁桃体におけるDbx1由来神経細胞の解析 (2013)
  • Author(s): 江角重行
  • Organizer: 熊本シンポジウム神経発生を多角的に討論する会
  • Place of Presentation: 熊本大学発生医学研究所、グリーンピア阿蘇
  • Year and Date: 20130625-26