2013 Fiscal Year Final Research Report
Genome-wide mapping of Msuashi1-RNA interaction
| Project/Area Number |
24700333
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Neuroscience in general
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
YANO Masato 慶應義塾大学, 医学部, 講師 (20445414)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | RNA結合蛋白質 / HITS-CLIP / 転写後調節機構 / 神経発生 |
|---|
| Research Abstract |
To understand a comprehensive role of neural RNA binding protein, Msi1, which is expressed in neural stem cells, we generated genome-wide Msi1-RNA binding map on the mouse embryonic brain by using HITS-CLIP, High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation methods. By using Msi1-RNA interaction map, we identified robust Msi1 RNA binding regions transcribed from the genome, specific in vivo RNA motif at the 1nt resolution, identified by CIMS assay and many novel RNA targets of Msi1. In addition to previously known Msi1 function, we found the novel functions of Msi1 in various aspects of RNA processing, validated in null mutant mice and gain of function study.
|
