2013 Fiscal Year Final Research Report
Analysis on the long association fibers in the cerebral cortex using a specific promoter
| Project/Area Number |
24700352
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Osaka University (2013)
University of Fukui (2012) |
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Principal Investigator |
OKA Yuichiro 大阪大学, 医学(系)研究科(研究院), 助教 (30614432)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 神経回路網 / 分子神経生物学 |
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| Research Abstract |
In this study, we identified the genes specifically expressed in the long association neurons connecting distant cortical areas in order to examine the detailed axon projection patterns and developmental processes of the circuit. First, we identified the genes expressed in the long association neurons by microarray analysis comparing gene expression profiles of the long association neurons and the callosal neurons, followed by double staining analysis combining retrograde tracing and in situ hybridization. We then used the promoters of these genes to drive the expression of the fluorescent reporter. We visualized the long association neurons and their axons by introducing the reporter constructs into the embryonic cortex and are currently analyzing the development of the neural circuit by examining the axonal patterns at different stages. For efficient observation and analysis, we also set up the tissue clearing procedure for the cortex using SeeDB method.
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Author(s)
Xie MJ, Yagi H, Kuroda K, Wang CC, Komada, M, Zhao H, Sakakibara A, Miyata T, Nagata K, Oka Y, Iguchi T, Sato M.
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Journal Title
Cereb.Cortex 2013
Volume: 23(6)
Pages: 1410-23
DOI
Peer Reviewed
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Author(s)
Toba S, Tamura Y, Kumamoto K, Yamada M, Takao K, Hattori S, Miyakawa T, Kataoka Y, Azuma M, Hayasaka K, Amamoto M, Tominaga K, Wynshaw-Boris A, Wanibuchi H, Oka Y, Sato M, Kato M, Hirotsune S.
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Journal Title
Sci Rep
Volume: 3
Pages: 1224
DOI
Peer Reviewed
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