Functional analysis of microRNA-124a which is highly expressed in the CNS

2013 Fiscal Year Final Research Report

• Project/Area Number: 24700360
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Osaka University
• Principal Investigator: SANUKI Rikako 大阪大学, たんぱく質研究所, 助教 (50607471)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: マイクロRNA / 中枢神経系 / 網膜 / アデノ随伴ウィルス

Research Abstract

MicroRNA-124a (miR-124a) is one of the most abundant microRNAs in the central nervous system (CNS). In order to clarify roles of miR-124a in the mature CNS, we are generating floxed miR-124a mice. We established the conditional gene deletion method upon Cre-recombinase expression in the retina and the brain using adeno-associated virus (AAV). Moreover, we identified candidate miR-124a target genes in the brain. The results in the current study contributed to progress of understanding on the miR-124a function in the CNS.

Research Products

• [Journal Article] Tropisms of AAV for subretinal delivery to the neonatal mouse retina and its application for in vivo rescue of developmental photoreceptor disorders (2013)
  • Author(s): Watanabe S, Sanuki R, Ueno S, Koyasu T, Hasegawa T, Furukawa T.
  • Journal Title: PLoS One Volume: 8(1) Pages: e54146
  • DOI: 10.1371/journal.pone.0054146
• [Journal Article] G9a histone methyltransferase activity in retinal progenitors is essential for proper differentiation and survival of mouse retinal cells (2012)
  • Author(s): Katoh K, Yamazaki R, Onishi A, Sanuki R, Furukawa T.
  • Journal Title: J Neurosci Volume: 32(49) Pages: 17658-17670
  • DOI: 10.1523/JNEUROSCI.1869-12.2012
• [Presentation] 網膜神経回路におけるシナプス位置制御による層構造形成メカニズム-Analysis of molecular mechanisms underlying layered neural network formation in the mouse retina (2013)
  • Author(s): 佐貫理佳子、杉田祐子、渡邉哲史、入江彰一、島田真理子、古川貴久
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2013-12-05
• [Presentation] アデノ随伴ウイルス(AAV)ベクターの血清型による網膜標的細胞の特異性の比較と網膜変性症モデルマウスのレスキューへの応用-Tropisms of AAV for Subretinal Delivery to the Neonatal Mouse Retina and Its Application for In Vivo Rescue of Developmental Photoreceptor Disorders (2013)
  • Author(s): 渡邉哲史、佐貫理佳子、上野真治、古川貴久
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2013-12-05
• [Presentation] 網膜シナプス層の位置決定の分子メカニズム (2013)
  • Author(s): 佐貫理佳子、渡邉哲史、入江彰一、古川貴久
  • Organizer: 平成25年度包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 名古屋国際会議場(名古屋市熱田区)
  • Year and Date: 2013-08-24
• [Presentation] 単一細胞ラベリング法によるマウス網膜層構造形成の観察 (2013)
  • Author(s): 小塚孝司,三浦隆義,佐貫理佳子,古川貴久
  • Organizer: 第17回視覚科学フォーラム
  • Place of Presentation: 立命館大学びわこ・くさつキャンパスローム記念館5階大会議室(草津市)
  • Year and Date: 2013-08-05
• [Presentation] 網膜視細胞で発現するSAMドメイン蛋白質の発現と機能の解析 (2013)
  • Author(s): 南卓矢,佐貫理佳子,古川貴久
  • Organizer: 第17回視覚科学フォーラム
  • Place of Presentation: 立命館大学びわこ・くさつキャンパスローム記念館5階大会議室(草津市)
  • Year and Date: 2013-08-05
• [Presentation] miR-124aは神経細胞の成熟と維持に関与する (2013)
  • Author(s): 佐貫理佳子,渡邉哲史,入江彰一,古川貴久
  • Organizer: 第15回日本RNA学会年会
  • Place of Presentation: 愛媛県民文化会館・ひめぎんホール(愛媛県・松山市)
  • Year and Date: 2013-07-24
• [Presentation] Micro RNA-124a is required for hippocampal axogenesis and retinal cone survival through Lhx2 suppression (2013)
  • Author(s): 入江彰一,佐貫理佳子,古川貴久
  • Organizer: 第8回研究所ネットワーク国際シンポジウム再生医学・再生医療の先端融合的共同研究拠点シンポジウム合同シンポジウム
  • Place of Presentation: 芝蘭会館稲盛ホール(京都市)
  • Year and Date: 2013-06-27
• [Presentation] G9a Histone Methyltransferase Activity in Retinal Progenitors is Essential for Proper Differentiation and Survival of Mouse Retinal Cells (2013)
  • Author(s): Ryoji Yamazaki, Kimiko Katoh, Rikako Sanuki, Takahisa Furukawa
  • Organizer: ARVO2013
  • Place of Presentation: Convention Center, Fort Lauderdale, Florida, USA
  • Year and Date: 2013-05-07
• [Presentation] Tropisms of AAV for Subretinal Delivery to the Neonatal Mouse Retina and Its Application for In Vivo Rescue of the Crx Knockout Retina (2013)
  • Author(s): Satoshi Watanabe, Rikako Sanuki, Shinji Ueno, Takahisa Furukawa
  • Organizer: ARVO2013
  • Place of Presentation: Convention Center, Fort Lauderdale, Florida, USA
  • Year and Date: 2013-05-07
• [Presentation] G9aヒストンメチルトランスフェレースによるエピジェネティック制御のマウス網膜発生における役割(An important role of epigenetic gene regulation by G9a histone methyltransferase in mouse retinal development) (2012)
  • Author(s): 山崎竜史,加藤君子,大西暁士,佐貫理佳子,古川貴久
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡(福岡市)
  • Year and Date: 2012-12-11
• [Presentation] マイクロRNA-124aの海馬神経細胞軸索形成と網膜錐体視細胞における機能(MicroRNA-124a regulates hippocampal axogenesis and retinal cone photoreceptor survival) (2012)
  • Author(s): 佐貫理佳子,渡邉哲史,古川貴久
  • Organizer: 第35回日本神経科学学会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市)
  • Year and Date: 2012-09-19
• [Presentation] RAX Homeoprotein and NOTCH-HES Signaling Regulate Otx2 Expression in Embryonic Retinal Photoreceptor Cell Fate Determination (2012)
  • Author(s): Takahisa Furukawa, Yuki Muranishi, Koji Terada, Tatsuya Inoue, Kimiko Katoh, Toshinori Tsujii, Rikako Sanuki, Yasuhiro Tamaki
  • Organizer: ARVO2012
  • Place of Presentation: Convention Center, Fort Lauderdale, Florida, USA
  • Year and Date: 2012-05-08
• [Book] MicroRNA let-7: Role in Human Diseases and Drug Discovery, Chapter 9"Roles of let-7 family miRNAs in development and differentiation" (2012)
  • Author(s): Sanuki R, Furukawa T.
  • Total Pages: 125-144
  • Publisher: Nova Science Publishers
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/furukawa_lab/