In vivo dendritic calcium imaging of a single neuron

2013 Fiscal Year Final Research Report

• Project/Area Number: 24700402
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: SATOSHI Manita 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (80584135)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 樹状突起 / カルシウム / in vivo

Research Abstract

We established calcium imaging methods from dendrites using a new multi-photon laser microscope. There is the progress of research on the following points using multiple techniques. We confirmed that the reciprocal projection between the primary somatosensory cortex (S1) and the secondary motor cortex (M2) in mouse. We revealed that the input from M2 causes firing of selective layer 5 neurons in the S1, which is following dendritic spikes. From behavioral experiments, which is related with haptic perception in mouse, we found that an inhibition of M2 inputs with the optogenetical methods disturbed the haptic perception. From this study, we have found that the dendritic calcium response is a building block of a basic component of the perception.

Research Products

• [Presentation] 二次運動野からのトップダウン入力による体性感覚の制御 (2013)
  • Author(s): 真仁田聡、鈴木崇之、松元崇、本間千尋、山田一之、太田桂輔、小田川摩耶、松原智恵、大倉正道、佐藤正晃、中井淳一、林康紀、Matthew E. Larkum、村山正宜
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2013-06-21
• [Presentation] マウス脳における皮質間の相互連絡 (2013)
  • Author(s): 真仁田聡、鈴木崇之、小田川摩耶、松原智恵、大倉正道、佐藤正晃、中井淳一、林康、村山正宜
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2013-03-28
• [Presentation] Corticocortical connections between the hindlimb region of the primary somatosensory cortex and the secondary motor cortex in mice (2012)
  • Author(s): S.MANITA, T.SUZUKI, M.ODAGAWA, M.INOUE, M.E.LARKUM, M.MURAYAMA
  • Organizer: 北米神経科学学会大会(Annual Meeting of Society for Neuroscience 2012)
  • Place of Presentation: Ernest N. Morial Convention Center in New Orleans, LA
  • Year and Date: 2012-10-15
• [Presentation] in vivoマウス脳における単一神経細胞樹状突起からのカルシウムイメージング (2012)
  • Author(s): 真仁田聡、村山正宜
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-09-19
• [Presentation] マウス大脳皮質における知覚に関与するトップダウン回路
  • Author(s): 真仁田聡,鈴木崇之,本間千尋,松元崇,小田川摩耶,山田一之,太田桂輔,松原智恵,犬束歩,佐藤正晃,大倉正道,山中章弘,柳川右千夫,中井淳一,林康紀, Matthew E. Larkum,村山正宜
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜