2013 Fiscal Year Final Research Report
In vivo two-photon imaging of learning-induced hippocampal neuronal circuit plasticity
| Project/Area Number |
24700403
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
SATO Masaaki 独立行政法人理化学研究所, 脳科学総合研究センター, 研究員 (90518325)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 脳・神経 / カルシウムイメージング / 二光子レーザー顕微鏡 / バーチャルリアリティ / マウス |
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| Research Abstract |
It has been thought that acquisition, retension and recall of memories are mediated by activity of groups of neurons that are formed and reorganized by learning and experience. In this study, we imaged hippocampal CA1 neuronal circuit activity in head-fixed mice performing spatial behavior in virtual reality by using two-photon deep brain imaging of transgenic mice expressing genetically-encoded calcium indicators in the brain. We computationally extracted activity time-series of hundreds of neurons from images and confirmed that a subpopulation of CA1 pyramidal neurons exhibited virtual place-specific activity. We also observed that neurons with different virtual place fields were anatomically intermingled each other in hippocampal CA1 local circuits.
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[Presentation] Hippocampal CA1 network dynamics during locomotion in virtual reality2013
Author(s)
Masaaki Sato, Kotaro Mizuta, Masako Kawano, Takashi Takekawa, Tanvir Islam, Hiroshi Yamakawa, Yoko Yamaguchi, Tomoki Fukai, Masamichi Ohkura, Junichi Nakai, Yasunori Hayashi
Organizer
Society for Neuroscience 43rd annual meeting
Place of Presentation
米国サンディエゴ
Year and Date
2013-11-13
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