Development of highly sensitive methods for phosphorylation analysis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24700407
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Asahikawa Medical College
• Principal Investigator: TAKEYA Kosuke 旭川医科大学, 医学部, 助教 (20586862)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 平滑筋 / 毛様体筋 / リン酸化 / ミオシン

Outline of Final Research Achievements

Bovine ciliary muscle has unique contractile properties characterized by rapid response to stimuli and sustained contraction. In this study, to address the molecular mechanisms of contraction, I performed phosphorylation analysis by using highly sensitive quantification methods which we have developed. In bovine ciliary muscle, no significant changes were observed in myosin light chain phosphorylation in carbachol-induced or ionomycin-induced contractions regardless of contraction and relaxation. Furthermore, inhibition of myosin light chain kinase could not attenuate contraction, suggesting that ciliary muscle has a unique regulatory mechanisms.

Free Research Field

生理学