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2013 Fiscal Year Final Research Report

Functional analysis of CTCF for generating neuronal diversity and neuronal networks

Research Project

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Project/Area Number 24700410
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Fusional basic brain science
Research InstitutionOsaka University

Principal Investigator

HIRAYAMA Teruyoshi  大阪大学, 生命機能研究科, 特任助教 (40437398)

Project Period (FY) 2012-04-01 – 2014-03-31
KeywordsCTCF / Neuron / diversity
Research Abstract

I generated postmitotic neurons specific CTCF conditional knockout mice and investigated the role of the brain and the regulation of clustered Pcdh genes. The CTCF-cKO mice exhibited growth retardation, abnormal behavior, and died within one month. Gene expression profile exhibited significant downregulation of clustered Protocadherin genes. I examined structure of somatosensory cortex and found that barrel structure was disappeared in the CTCF-cKO mice. I also found that the CTCF deficiency lead to morphological changes of dendrite and spine formation during neuronal maturation. These results indicate that CTCF is required for the refinement of somatosensory maps and stochastic expression of clustered Pcdh genes in neurons.

  • Research Products

    (10 results)

All 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (7 results)

  • [Journal Article] Developmental epigenetic modification regulates stochastic expression of clustered Protocadherin genes, generating single neuron diversity2014

    • Author(s)
      Toyoda S, Kawaguchi M, Kobayashi T, Tarusawa E, Toyama T, Okano M, Oda M, Nakauchi H, Yoshimura Y, Makoto S, Hirabayashi M, Hirayama T, Hirabayashi T, Yagi T
    • Journal Title

      Neuron.

      Volume: 82 Pages: 94-108

    • DOI

      10.1016/j.neuron.2014.02.005

    • Peer Reviewed
  • [Journal Article] Clustered Protocadherins and Neuronal Diversity2013

    • Author(s)
      Hirayama T and Yagi T
    • Journal Title

      Molecular Biology and Translational Science(In Frans van Roy, editor)

      Volume: Vol. 116(Elsevier Academic Press) Pages: 145-167

    • DOI

      10.1016/B978-0-12-394311-8.00007-8

  • [Journal Article] CTCF Is Required for Neural Development and Stochastic Expression of Clustered Pcdh Genes in Neurons2012

    • Author(s)
      Hirayama T, Tarusawa E, Yoshimura Y, Galjart N, Yagi T
    • Journal Title

      Cell Rep.

      Volume: 2 Pages: 345-357

    • DOI

      10.1016/j.celrep.2012.06.014

    • Peer Reviewed
  • [Presentation] Behavior analysis of CTCF-deficient mice specifically in Purkinje cell2013

    • Author(s)
      角岡佑紀、平山晃斉、崎村建司、八木健
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      20131203-06
  • [Presentation] CTCF is required for the refinement of somatosensory maps and stochastic expression of clustered Pcdh genes in neurons2013

    • Author(s)
      Hirayama T, Tarusawa E, Yoshimura Y, Galjart N, Yagi T
    • Organizer
      EMBO meeting 2013
    • Place of Presentation
      Amsterdam (Netherlands)
    • Year and Date
      20130921-24
  • [Presentation] Dnmt3b依存的なDNAメチル化は単一神経細胞におけるクラスター型プロトカドヘリン遺伝子群の確率的発現と樹状突起の自己忌避を制御する2013

    • Author(s)
      豊田峻輔、川口将史、小林俊寛、足澤悦子、遠山知子、岡野正樹、小田昌朗、中内啓光、吉村由美子、三宝誠、平林真澄、平山晃斉、平林敬浩、八木健
    • Organizer
      第36回日本神経科学学会
    • Place of Presentation
      京都
    • Year and Date
      20130620-23
  • [Presentation] DNAポリメラーゼβの神経前駆体細胞における作用が大脳皮質における神経細胞分化に必要である2013

    • Author(s)
      大西公平、菅生紀之、豊田峻輔、平山晃斉、八木健、山本亘彦
    • Organizer
      第36回日本神経科学学会
    • Place of Presentation
      京都
    • Year and Date
      20130620-23
  • [Presentation] CTCFによる神経細胞の多様化と神経回路形成2013

    • Author(s)
      平山晃斉、足澤悦子、吉村由美子、Galjart N、八木健
    • Organizer
      第7回日本エピジェネティック研究会年会
    • Place of Presentation
      奈良
    • Year and Date
      20130530-31
  • [Presentation] CTCF is required for neural development and stochastic expression of clustered Pcdh genes in neurons2012

    • Author(s)
      Hirayama T, Tarusawa E, Yoshimura Y, Galjart N, Yagi T
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      博多
    • Year and Date
      20121211-14
  • [Presentation] Promoter DNA methylation is dependent on gene cluster structure and regulates allocation of isoforms expression in each Protocadherin cluster2012

    • Author(s)
      Toyoda S, Kawaguchi M, Toyama T, Okano M, Oda M, Kobayashi T, Hirayama T, Hirabayashi T, Yagi T
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      20120918-21

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Published: 2015-06-25  

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