Establishment of mouse infection model of HCV

2014 Fiscal Year Final Research Report

• Project/Area Number: 24700429
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Osaka University
• Principal Investigator: ONO Chikako 大阪大学, 微生物病研究所, 特任研究員(常勤) (30626437)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: C型肝炎ウイルス / 感染症 / マウスモデル

Outline of Final Research Achievements

We established mouse immortalized hepatocyte cell lines that stably expresses four HCV receptor candidates (hCD81, hSR-B1, hCLDN-1, hOCLN) and liver-specific micro RNA, miR-122. Although this cell line permits HCV entry and slight replication of HCV-RNA and virus protein expression were observed upon infection with HCV, efficient replication of HCV-RNA and production of infectious particles were not detected. Then, we could not obtain mouse-adapted HCV that can propagate efficiently in mouse cell lines.

Free Research Field

肝炎ウイルス学