2013 Fiscal Year Final Research Report
Studies on turnover regulation of mitochondrial DNA in mice and methodological improvements for its quantitative analysis
| Project/Area Number |
24700442
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
SHIMANUKI Midori 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 基盤技術研究職員 (20593643)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ミトコンドリア / ミトコンドリアDNA / mtDNA / コピー数 / 遺伝学 |
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| Research Abstract |
In this study, we aimed to understand the mechanism that regulates the turnover of mitochondrial DNA (mtDNA), an extra-nuclear genome. First, we demonstrated that the quantity of mtDNA differs among mouse tissues that differ in metabolic properties using real-time PCR. To investigate the factors regulating mtDNA copy number, we temporarily fasted mice to alter the physiological environment. Following fasting, the quantity of mtDNA decreased in some tissues. However, expression of several genes that positively regulate mtDNA copy number increased in most tissues, and the intracellular degradation machinery was activated in the fasted state. These observations indicate that autophagy may be associated with turnover of mtDNA.
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[Journal Article] p62 /SQSTM1 cooperates with Parkin for perinuclear clustering of depolarized mitochondria2010
Author(s)
Okatsu K, Saisho K, Shimanuki M, Nakada K, Shitara H, Sou YS, Kimura M, Sato S, Hattori N, Komatsu M, Tanaka K, and Matsuda N
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Journal Title
Genes to Cells
Volume: 15(8)
Pages: 887-900
Peer Reviewed
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