2013 Fiscal Year Final Research Report
Development of enzyme-loaded nano-sized polyion complex vesicles (PICsomes) for enzyme prodrug therapy
| Project/Area Number |
24700476
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ANRAKU Yasutaka 東京大学, 工学(系)研究科(研究院), 助教 (60581585)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | Enzyme/Prodrug Therapy / ナノリアクター / PICsome / 制がん剤 |
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| Research Abstract |
Utilization of enzymes in the body is still a big challenge in the biomedical field. One of promising applications of enzymes for therapeutic application is enzyme prodrug therapy, and development of nano-vehicles for enzymes is one effective clue to establish the golden standard of EPT. We succeed in the preparation of b-galactosidase loaded PICsome with the diameter of 100 nm, maintaining enzyme activity. Exploiting b-gal-loaded PICsomes as an enzyme vehicles, a model prodrug, HMDER-bGAL, was successfully converted into highly fluorescent product, HMDER, due to permeation of HMDER-bGAL through the vesicle wall. In in vivo experiments using tumor-bearing mice, b-gal-loaded PICsomes were accumulated in the tumor tissue, to produce HMDER. Resulted HMDER was released form PICsomes and distributed into the entire tumor tissues and internalized by cells, and then visualization of tumor tissues was performed.
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Research Products
(21 results)
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[Journal Article] Polyion complex vesicles for photo-induced intracellular delivery of amphiphilic photosensitizer2014
Author(s)
H. Chen, L. Xiao, Y. Anraku, P. Mi, X. Liu, H. Cabral, A. Inoue, T. Nomoto, A. Kishimura, N. Nishiyama, K. Kataoka
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Journal Title
J. Am. Chem. Soc.
Volume: 136 (1)
Pages: 157-163
DOI
Peer Reviewed
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[Presentation] Development of enzyme-loaded nano-sized polyion complex vesicles (PICsomes) for enzyme prodrug therapy FIRST合同国際シンポジウム難治がんを克服する―次世代の診断、治療が描く未来2014
Author(s)
Y. Anraku, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, S. Fukushima, A. Kishimura, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
Organizer
Overcoming Intractable Cancer- a new path to the future
Place of Presentation
伊藤国際学術研究センター文京区、東京都
Year and Date
2014-02-21
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[Presentation] Development of enzyme-loaded nano- sized polyion complex vesicles (PICsomes) for enzyme/prodrug therapy2013
Author(s)
Y. Anraku, A. Kishimura, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
Organizer
第35回日本バイオマテリアル学会大会
Place of Presentation
タワーホール船堀、江戸川、東京
Year and Date
2013-11-25
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[Presentation] Development of enzyme-loaded PICsomes for enzyme2013
Author(s)
Y. Anraku, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, A. Kishimura, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
Organizer
prodrug therapy 40th Annual Meeting & Exposition of the Controlled Release Society
Place of Presentation
Hawaii Convention Center, Honolulu, Hawaii, USA
Year and Date
2013-07-21
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[Presentation] Development of nano-sized polyion complex vesicles (Nano-PICsomes) as a versatile delivery carrier for biomedical application FRONTIERS2013 - Joint EPFL2013
Author(s)
Y. Anraku, A. Kishimura, D. Kokuryo, S. Tanaka, M. Kamiya, K. Tou, T. Nomoto, Y. Matsumoto, I. Aoki, M. Kano, Y. Urano, K. Kataoka
Organizer
University of Tokyo Symposium on "Frontiers in Nanomedicine and Imaging" Swiss Federal Institute of Technology
Place of Presentation
Lausanne, Switzerland
Year and Date
2013-06-21
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[Presentation] Development of nano-sized polyion complex vesicles (Nano-PICsomes) for versatile DDS carriers2012
Author(s)
Y. Anraku, A. Kishimura, D. Kokuryo, S. Tanaka, M. Kamiya, K. Toh, T. Nomoto, Y. Matsumoto, I. Aoki, M. Kano, Y. Urano, K. Kataoka
Organizer
2012 MRS Fall Meeting
Place of Presentation
Hynes Convention Center, Boston, Massachusetts, USA
Year and Date
2012-11-26
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