2013 Fiscal Year Final Research Report
Control of cellular immunity by multi-step antigenic peptide delivery using peptide nano-needles
| Project/Area Number |
24700479
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
WAKU Tomonori 京都工芸繊維大学, 工芸科学研究科, 助教 (30548699)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ペプチド / 免疫 / ドラッグデリバリーシステム / ナノ粒子 / 自己組織化 |
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| Research Abstract |
Synthetic immunogenic peptides are ideal vaccine subunit components for cancer immunotherapy due to advances in safety, stability, and design. Cytoplasmic delivery system of antigenic peptides has been required for effective cancer immunotherapy. In this study, we develop the direct cytoplasmic delivery system of antigenic peptides by using beta-sheet peptide nano-needles as carriers. We investigated the effect of nano-needle length on their cellular uptake by RAW264 cells and the cellular internalization pathway. The amount of cellular uptake was significantly affected by the length of the nano-needles. Interestingly, the short nano-needles with a length of 40 nm was internalized by non-endocytosis pathway, in contrast that the longer nano-needles with a length of 120 nm - 800 nm via endocytosis pathway. The size-regulated peptide nano-needles are expected to be useful as a novel delivery carrier of antigenic peptides into cytoplasm of antigen-presenting cells.
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