2013 Fiscal Year Final Research Report
Development of biomolecule separation system by hybrid coacervate using hydraulic pressure
| Project/Area Number |
24700483
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | インテリジェント材料 / コアセルベート |
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| Research Abstract |
We examined the development of stimuli-responsive hybrid coacervate as the reaction field under high pressure condition by hydraulic pressure. First, we designed the acrylamide-based copolymers with higher molecular weight randomly containing carboxyl or hydroxyl groups. Their stimuli-responsive behavior on comonomer composition and pH of the polymer solution was precisely investigated. The coacervate droplets were easily formed above a lower critical solution temperature. Furthermore, we studied the coacervate droplet formation under high pressure conditions. Next, we examined radical polymerization and biomolecule separation system with the coacervate droplet under high pressure. When we added the ferritin protein cages (ca. 12 nm) as model biomolecule to the coacervate droplets, the composite emulsions were stable for a long time. Furthermore, we examined the assembly behavior of ferritin on the liquid-liquid and liquid-solid interface.
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