2014 Fiscal Year Final Research Report
Development of a New Cryopreservation Method Using Microdroplet Liquid
| Project/Area Number |
24700500
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical systems
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| Research Institution | Miyagi University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 凍結保存 |
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| Outline of Final Research Achievements |
We developed a new cryopreservation method where living organisms to be frozen are suspended in silicon oil. When processed in suspension in this manner, living organisms in a 100-micrometer-order size, for example, were found to be maintained in a supercooled state down to nearly -20°C. Furthermore, this suspended processing followed by slow freezing tended to increase the survival rate compared to the processing without slow freezing. Besides, as a new cryopreservation method, we also developed a device for freezing living organisms as solid particles in an approximate minimum size of 100 micrometer.
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| Free Research Field |
生体および食品の保存
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