2014 Fiscal Year Final Research Report
Establishment of neural factor for regulating exercise activity and cardiac hypertrophy
| Project/Area Number |
24700754
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 脳内モノアミン / 心肥大 / 運動習慣 / 自発運動 |
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| Outline of Final Research Achievements |
We established a new animal model called SPORTS rats. The aim of this study was to investigate the role of the L-type Ca2+ channel in cardiomyocytes and its relation with intracellular Ca2+-dependent signalings in cardiac adaptation responding to high physical activity. Injection of Ca2+ channel antagonist verapamil markedly decreased wheel-running activity with a reduction of heart rate in SPORTS rats. In isolated neonatal rat cardiomyocytes from SPORTS rats, spontaneous beating rate, L-type Ca2+ current, and the expression ofits mRNAs were significantly higher than those in control rat. Phosphorylation of CREB and Akt were significantly elevated in SPORTS rat myocytes in the basal condition. These results suggest that phosphorylation of the CREB via activation of PI3K/Akt signaling pathway in SPORTS rats positively regulates the L-type Ca2+ channel expression, which is responsible for the enhancement of physiological hypertrophy and chronotropy in the heart.
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| Free Research Field |
運動生理学
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