2013 Fiscal Year Annual Research Report
ミトコンドリア特異的一重項酸素の消去による光老化の予防
| Project/Area Number |
24700762
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| Research Institution | Nippon Medical School |
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Principal Investigator |
WOLF Alexander 日本医科大学, 付置研究所, 講師 (20434136)
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| Keywords | 光老化 / 老化 / 酸化ストレス / 皮膚 / skin / oxidative stress / aging |
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| Research Abstract |
Due to a high desire to look young and beautiful, cosmetics to slow down aging are a large market. Wrinkle formation has been attributed to UVA radiation.
Using hairless transgenic mice expressing a redox-sensitive GFP (roGFP1) in the cytosol and in mitochondria of epidermal keratinocytes, we discovered that UVA induces oxidative stress in mitochondria but not cytosol of live mouse epidermis . We used in vivo oxidative stress imaging to evaluate singlet oxygen scavengers to prevent the aging-like changes in UVA-exposed mouse skin. Topical application of carotenoids did not prevent UVA-induced oxidative stress. We assume this was due to poor permeation of the large carotenoids across the stratum corneum. However, a low molecular weight compound able to permeate the stratum corneum well could reduce the amount of oxidation induced by UVA relative to vehicle control.
We also investigated epidermal redox state in response to visible light of varying wavelength and intensity, and found that blue light could induce oxidative stress in live mouse skin. The efficacy of blue light to induce oxidative stress at an equal flux was lower than that of UVA light. These findings could be confirmed in human keratinocyte cell cultures exposed to UVA or blue light in vitro.
Future research will further investigate substances that can prevent UVA or visible light-induced oxidative stress by topical application to skin.
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