2014 Fiscal Year Final Research Report
The role of miRNAs in hydrostatic pressure-stimulated osteocyte and osteoblast.
| Project/Area Number |
24700767
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | メカニカルストレス / microRNA / 骨代謝 / 骨芽細胞 / 骨細胞 |
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| Outline of Final Research Achievements |
MC3T3-E1 cells were attached on biodegradable polymer scaffold and then cultured for 3 days. To investigate the expression profiles of miRNAs in hydrostatic pressure-stimulated MC3T3-E1 cells, we subjected their total RNA extracted from hydrostatic pressure-stimulated (1.5 MPa for 1 h) or nontreated cells to miRNA array chips. The expression levels of 120 miRNAs (log ratio 3 <) were distinct between hydrostatic pressure-stimulated and nontreated cells. Among them, we focused on the 3 kinds of down-regulated miRNAs, because the nucleotide sequences of these miRNAs were similar and the semaphorin 3A that is closely related in osteoblast differentiation was a common target gene for each miRNAs. The enforced expression of these mature miRNAs in MC3T3-E1 cells remarkably attenuated a hydrostatic pressure-stimulated preosteoblast differentiation. From these results, it was presumed that those miRNAs act as important factors for regulatory machinery involved in early osteogenesis in part.
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| Free Research Field |
食品機能学, 骨代謝化学
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