A new method to evaluate parytoxin content in sea foods

2014 Fiscal Year Final Research Report

• Project/Area Number: 24700857
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits, studies on eating habits
• Research Institution: Shokei Junior College
• Principal Investigator: SAGARA Takefumi 尚絅大学短期大学部, その他部局等, 講師 (60353132)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: パリトキシン / C6グリオーマ細胞 / PC12細胞 / LC-MS / アオブダイ / ハコフグ

Outline of Final Research Achievements

In this project, I investigated to develop a new method using cultured cell to evaluate palytoxin content. As the results, a rat C6 glioma cell was suitable to use for a toxic evaluation for the palytoxin, furthermore I conclude that the method of detection by LDH release was sensitive. In addition, the method was applicable to the test solution that I prepared from toxic specimen Scarus ovifrons. On the other hand, peaks were detected in chromatogram of m/z 327.6, 1352.3 and 1360.3 in LC-MS analysis of the test solution. However did not come to conclude that it was the peak to originate from palytoxin or a palytoxin-like substance because the influence was inarticulate peak shape.

Free Research Field

食品学