2013 Fiscal Year Final Research Report
Identification of Ral kinase and characterization of its role in tumorigenesis
| Project/Area Number |
24700961
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | Ral / 低分子量GTP結合蛋白質 |
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| Research Abstract |
The small GTPase RalA regulates many cellular processes such as cell proliferation, migration, membrane traffic, and tumorigenesis. Accumulating evidence shows that activation of RalA GTPase is essential for oncogenic Ras-induced human cell tumorigenesis. However, the molecular mechanism by which RalA regulates tumorigenesis remains elusive. I tried to identify RalA effector proteins in rat brain cytosol and succeeded in purifying a RalA effector complex using several chromatography steps including ammonium sulphate precipitation, ion-exchange chromatograhpy, and RalA affinity chromatography. This effector complex may be involved in RalA-dependent tumorigenesis.
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[Journal Article] Downregulation of Ral GTPase-activating protein promotes tumor invasion and metastasis of bladder cancer2013
Author(s)
Saito R, Shirakawa R, Nishiyama H, Kobayashi T, Kawato M, Kanno T, Nishizawa K, Matsui Y, Ohbayashi T, Horiguchi M, Nakamura T, Ikeda T, Yamane K, Nakayama E, Nakamura E, Toda Y, Kimura T, Kita T, Ogawa O, Horiuchi H
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Journal Title
Oncogene
Volume: 32巻
Pages: 894-902
DOI
Peer Reviewed
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