2013 Fiscal Year Final Research Report
Comprehensive metabolome analysis with high resolution and sensitve system
| Project/Area Number |
24701005
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnosis
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| Research Institution | Kobe University |
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Principal Investigator |
IRINO Yasuhiro 神戸大学, 医学(系)研究科(研究院), 特命助教 (10415565)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 代謝 / メタボローム解析 |
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| Research Abstract |
Metabolomics is a powerful approach to discover new biomarkers of various diseases. In this study, we tried to develop the high sensitive and resolution metabolome analysis system.
We first focused on a tumor suppressor gene, Adenomatous polyposis coli (APC). We conducted the comprehensive quantification of water-soluble metabolites and proteins in colon cancer cells (both APC mutation cell and APC wild-type cell) using a mass spectrometry. We determined target metabolites for our system. We also found the levels of some metabolic enzymes were regulated by APC activity. These results suggest that APC mutation causes metabolite pathways in colon cancers.
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Research Products
(5 results)
