2013 Fiscal Year Final Research Report
Gene expression analysis of retinoic acid-responsive genes in hepatocellular carcinoma
| Project/Area Number |
24701006
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnosis
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| Research Institution | Tottori University |
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Principal Investigator |
KANKI Keita 鳥取大学, 医学(系)研究科(研究院), 助教 (10516876)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 肝細胞癌 / レチノイド |
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| Research Abstract |
We performed a gene screening based on in silico analysis of retinoic acid response elements (RAREs) to identify the genes facilitating the antitumor activity of retinoic acid (RA) and investigated their clinical significance in hepatocellular carcinoma (HCC). We identified 201 candidate genes with promoter regions containing consensus RARE and finally selected 26 RAresponsive genes. Of these, downregulation of OTU domain-containing 7B (OTUD7B) gene, which was upregulated by ATRA, in tumor tissue was associated with a low cancer-specific survival of HCC patients. Functional analyses revealed that OTUD7B negatively regulates nuclear factor jB (NF-jB) signaling and decreases the survival of HCC cells. We identified RA-responsive genes which are regulated by retinoid signal and found that low-OTUD7B mRNA expression is associated with a poor prognosis for HCC patients. OTUD7B-mediated inhibition of NF-jB signaling may be an effective target for antitumor therapy for HCC.
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[Journal Article] Biological and clinical implicationsof retinoic acid-responsive genes in human hepatocellular carcinoma cells2013
Author(s)
Kanki K, Akechi Y, Ueda C, Tsuchiya H, Shimizu H, Ishijima N, Toriguchi K,Hatano E, Endo K, Hirooka Y, Shiota G
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Journal Title
Journal of Hepatology
Volume: 59
Pages: 1037-44
Peer Reviewed
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