2013 Fiscal Year Final Research Report
Basic research for early diagnosis of lung carcinomas by the application of DNA methylation analysis
| Project/Area Number |
24701008
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnosis
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| Research Institution | Kagoshima University |
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Principal Investigator |
YOKOYAMA Seiya 鹿児島大学, 医歯(薬)学総合研究科, 助教 (20569941)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | lung adenocarcinomas / mucin / DNA methylation / demethylation / ten eleven translocation |
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| Research Abstract |
Lung cancer is still a disease of high mortality, despite advanced diagnostic techniques. Mucins (MUC) play crucial roles in carcinogenesis and tumor invasion in lung neoplasms. We have developed a novel 'methylation-specific electrophoresis (MSE)' method to analyze the DNA methylation status of MUC1 and MUC4 by high sensitivity and resolution.
By using the MSE method, we evaluated lung tissue samples from 33 patients with various lung lesions. Our results showed that the TET1 was important factor for DNA demethylation to regulate the expression of MUC1 and MUC4 in lung cancer. The analysis of the epigenetic changes of MUC1 and MUC4 may be useful for diagnosing the carcinogenic risk and predicting outcome of patients.
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[Journal Article] Diagnosis of pancreatic neoplasms using a novel method of DNA methylation analysis of mucin expression in pancreatic juice2014
Author(s)
Yokoyama S, Kitamoto S, Higashi M, Goto Y, Hara T, Ikebe D, Yamaguchi T, Arisaka Y, Niihara, Nishimata H, Tanaka S, Takaori K, Batra S K, Yonezawa S
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Journal Title
PLoS ONE
Volume: 9(4)
Pages: e93760
DOI
Peer Reviewed
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[Journal Article] Pathobiological implications of mucin (MUC) expression in the outcome of small bowel cancer2014
Author(s)
Shibahara H, Higashi M, Koriyama C, Yokoyama S, Kitazono I, Yasuhiro K, Narita M, Kuze S, Takanori K, Mita S, Arai T, Kato T, Yuasa N, Yamaguchi R, Kubota H, Suzuki H, Baba S, Rousseau K, Batra S K, Yonezawa S
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Journal Title
PLoS ONE
Volume: 9(4)
Pages: e86111
DOI
Peer Reviewed
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[Journal Article] Expression of MUC4 mucin is observed mainly in the intestinal-type of intraductal papillary mucinous neoplasm of the pancreas2013
Author(s)
Kitazono I, Higashi M, Kitamoto S, Yokoyama S, Horinouchi M, Osako M, Shimizu T, Tabata M, Batra S K, Goto M, Yonezawa S
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Journal Title
Pancreas
Volume: 42(7)
Pages: 1120-1128
DOI
Peer Reviewed
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[Journal Article] MUC4 and MUC1 expression in adenocarcinoma of the stomach correlates with vessel invasion and lymph node metastasis : an immunohistochemical study of early gastric cancer2012
Author(s)
Tamura Y, Higashi M, Kitamoto S, Yokoyama S, Osako M, Horinouchi M, Shimizu T, Tabata M, Batra SK, Goto M, Yonezawa S
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Journal Title
PLoS ONE
Volume: 7(11)
Pages: e49251
DOI
Peer Reviewed
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[Journal Article] A novel anti-MUC1 antibody against the MUC1 cytoplasmic tail domain : use in sensitive identification of poorly differentiated cells in adenocarcinoma of the stomach2012
Author(s)
Yonezawa S, Kitajima S, Higashi M, Osako M, Horinouchi M, Yokoyama S, Kitamoto S, Yamada N, Tamura Y, Shimizu T, Tabata M, Goto M
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Journal Title
Gastric Cancer
Volume: 15(4)
Pages: 370-381
DOI
Peer Reviewed
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[Presentation] The Application of New DNA Methylation Analysis, Designated Methylation Specific Electrophoresis (MSE) for Pancreatic Juice Analysis2012
Author(s)
Yokoyama S, kitamoto S, Hara T, Arisaka Y, Takaori K, Yamamoto T, Niihara T, Nishimata H, Tanaka S, Higashi M, Yonezawa S
Place of Presentation
京都国際コンベンションセンター(京都)
Year and Date
20121004-06
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