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2013 Fiscal Year Final Research Report

Detection of EML4-ALK in serum RNA from lung cancer patients

Research Project

  • PDF
Project/Area Number 24701013
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor diagnosis
Research InstitutionKinki University

Principal Investigator

SAKAI Kazuko  近畿大学, 医学部, 助教 (20580559)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywords薬効評価と予測
Research Abstract

The presence of the transforming fusion gene EML4-ALK in non–small cell lung cancer (NSCLC) is a predictive marker for the efficacy of ALK kinase inhibitors. Circulating RNA in serum is an emerging field for noninvasive molecular diagnosis. We designed EML4-ALK detection assay using PCR-based MassARRAY platform. We attempted a RNA extraction method, primer design, and reverse transcription reagents to optimize our detection system with serum samples. We evaluated whether EML4-ALK fusion RNA could be detected in serum obtained from 12 NSCLC patients with ALK rearrangements. The EML4-ALK RNA (variant 1 and 3) was detected in serum from 2 of 12 patients. The detection of EML4-ALK in serum RNA samples by MassARRAY assay is feasible. This noninvasive assay will be useful in patients with insufficient or unavailable tumor specimens. Additional experiments will reveal the sensitivity and specificity of this detection system.

  • Research Products

    (6 results)

All 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (3 results)

  • [Journal Article] Detection of EGFR T790M mutation in plasma DNA from patients2013

    • Author(s)
      Sakai, K., Horiike, A., Darryl, I., Keita, K., Fujita, Y., Tanimoto, A., Sakatani, T., Saito, R., Kaburaki, K., Noriko, Y., Ohyanagi, F., Nishio, M., Nishio, K.
    • Journal Title

      Cancer Sci

      Volume: 104(9) Pages: 1198-204

    • Peer Reviewed
  • [Journal Article] Clinical outcome for EML4-ALK-positive patients with advanced non-small-cell lung cancer treated with first-line platinum-based chemotherapy2012

    • Author(s)
      Takeda, M., Okamoto, I., Sakai, K., Kawakami, H., Nishio, K., Nakagawa, K.
    • Journal Title

      Ann Oncol

      Volume: 23(11) Pages: 2931-6

    • Peer Reviewed
  • [Journal Article] A novel mass spectrometry-based assay for EML4 exon 13 ALK exon 20 EML4 exon 6 ALK exon 20 diagnosis of EML4-ALK-positive non-small cell lung cancer2012

    • Author(s)
      Sakai, K., Okamoto, I., Takezawa, K., Hirashima, T., Kaneda, H., Takeda, M., Matsumoto, K., Kimura, H., Fujita, Y., Nakagawa, K., Arao, T., Nishio, K.
    • Journal Title

      J Thorac Oncol

      Volume: 7(5) Pages: 913-8

    • Peer Reviewed
  • [Presentation] Detection of EGFR T790M mutation in plasma DNA from patients refractory to EGFR tyrosine kinase inhibitor2013

    • Author(s)
      Sakai, K., Horiike, A., Darryl, I., Keita, K., Fujita, Y., Tanimoto, A., Sakatani, T., Saito, R., Kaburaki, K., Noriko, Y., Ohyanagi, F., Nishio, M., Nishio, K.
    • Organizer
      The 11th Annual Meeting of Japanese Society of Medical Oncology
    • Place of Presentation
      Sendai, JAPAN
    • Year and Date
      20130829-31
  • [Presentation] A novel mass spectrometry-based assay for diagnosis of EML4-ALK-positive non-small cell lung cancer2012

    • Author(s)
      Sakai, K., Okamoto, I., Takezawa, K., Hirashima, T., Kaneda, H., Takeda, M., Matsumoto, K., Kimura, H., Fujita, Y., Nakagawa, K., Arao, T., Nishio, K.
    • Organizer
      The 10th Annual Meeting of Japanese Society of Medical Oncology
    • Place of Presentation
      Osaka, JAPAN
    • Year and Date
      20120726-28
  • [Presentation] Detection of EML4-ALK in serum RNA from lung cancer patients using MassARRAY platform2012

    • Author(s)
      Kudo, K., Nishio, M., Sakai, K., Tanimoto, A., Sakatani, T., Saito, R., Kaburaki, K., Yanagitani, N., Horiike, A., Ohyanagi, F., Nishio, K.
    • Organizer
      ASCO annual Meeting
    • Place of Presentation
      Chicago, USA
    • Year and Date
      20120601-05

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Published: 2015-06-25  

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