2013 Fiscal Year Final Research Report
Development of high-throughput screening system using autoantibody phage library for finding novel diagnostic biomarker of gastric cancer
| Project/Area Number |
24701015
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnosis
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| Research Institution | 独立行政法人医薬基盤研究所 |
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Principal Investigator |
MURAOKA SATOSHI 独立行政法人医薬基盤研究所, 創薬基盤研究部, 研究員 (50582681)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 抗体ファージ |
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| Research Abstract |
We report on high-throughput screening system using autoantibody phage library for finding novel diagnostic biomarker of gastric cancer. The antibody phage library was constructed from peripheral blood lymphocytes from gastric cancer patients using polymerase chain reaction. We panned the constructed library with scirrhous gastric cancer and disease-free control serum to make a sublibrary of antibodies that bind proteins differentially expressed, structurally changed, and post-translationally modified. To confirm this, we examined the binding ability of enriched antibody phage using ELISA. The results indicated that they were binding against gastric cancer serum. In the future, we will perform to isolate antibody phage clone specific to gastric cancer serum.
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Research Products
(14 results)
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[Journal Article] A Strategy for Large-Scale Phosphoproteomics and SRM-Based Validation of Human Breast Cancer T issue Samples2012
Author(s)
Ryohei Narumi, Tatsuo Murakami, Takahisa Kuga, Jun Adachi, Takashi Shiromizu, Satoshi Muraoka, Hideaki Kume, Yoshio Kodera, Masaki Matsumoto, Keiichi Nakayama, Yasuhide Miyamoto, Makoto Ishitobi, Hideo Inaji, Kikuya Kato, and Takeshi Tomonaga
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Journal Title
J. Proteome Res.
Volume: 11 (11) (Peer-Reviewed)
Pages: 5311–22
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[Journal Article] Strategy for SRM-based Verification of Biomarker Candidates Discovered by iTRAQ Method in Limited Breast Cancer Tissue Samples2012
Author(s)
Satoshi Muraoka, Hideaki Kume, Shio Watanabe, Masayoshi Kuwano, Misako Sato, Naoko Kawasaki, Jun Adachi, Makoto Ishitobi, Hideo Inaji, Yoshio Kodera, Yasuhide Miyamoto, Kikuya Kato, Takeshi Tomonaga
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Journal Title
J Proteome Res.
Volume: 11 (8) (Peer-Reviewed)
Pages: 4201-10
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[Presentation] A strategy for SRM-based systematic validation of biomarker candidates discovered by iTRAQ method in breast cancer tissue samples2012
Author(s)
Satoshi Muraoka, Hideaki Kume, Shio Watanabe, Masayoshi Kuwano, Misako Sato, Naoko Kawasaki, Jun Adachi, Makoto Ishitobi, Hideo Inaji, Yasuhide Miyamoto, Kikuya Kato, Yoshio Kodera, Takeshi Tomonaga
Organizer
HUPO 11th Annual World Congress
Year and Date
20120909-13
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[Presentation] A strategy for SRM-based large-scale validation of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples2012
Author(s)
Satoshi Muraoka, Hideaki Kume, Shio Watanabe, Masayoshi Kuwano, Misako Sato, Naoko Kawasaki, Jun Adachi, Makoto Ishitobi, Hideo Inaji, Yasuhide Miyamoto, Kikuya Kato, Yoshio Kodera, Takeshi Tomonaga
Organizer
Asia Oceania Human proteome organization 6^<th> Congress
Place of Presentation
Beijing China
Year and Date
20120505-07
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