Paleoenvironment reconstruction using the genetic information of a single pollen grain from ice cores

2014 Fiscal Year Final Research Report

• Project/Area Number: 24710019
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental dynamic analysis
• Research Institution: 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ
• Principal Investigator: NAKAZAWA Fumio 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ, 大学共同利用機関等の部局等, 研究員 (80432178)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 氷河 / 花粉 / ＤＮＡ / アイスコア / マツ属 / WGA

Outline of Final Research Achievements

This study developed new method to identify the pollen grains in ice cores at the species level by whole genome amplification method that may provide sufficient sequence data for the identification. Pollen grains used in this study were Pinus extracted from a ice core drilled from the Belukha glacier in the Altai Mountains of Russia in the summer of 2003. The developed new method was performed using a single pollen grain. The pollen grains from Belukha glacier were identified as P. Sylvestris, which are currently found at the periphery of the glacier. In addition, the DNA analyses indicated that P. Sylvestris is a dominant species in genus Pinus in this region.

Free Research Field

雪氷学