Research on the mechanism of radiation-induced brain dysfunction and the defense mechanism

2015 Fiscal Year Final Research Report

• Project/Area Number: 24710060
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyushu University
• Principal Investigator: AKIMOTO Yoriko 九州大学, 生体防御医学研究所, 特別研究員 (50613254)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: X線 / 修飾ヌクレオシド

Outline of Final Research Achievements

It is largely unknown whether modification of nucleotides by radiation contributes to its biological effects. In the present study, we comprehensively analyzed modification of purine nucleosides caused by X-ray irradiation. Using HPLC-PDA and LC-MS/MS systems, various forms of modified purine nucleosides in free purine nucleosides, DNA and RNA exposed to X-ray were identified in quantitative manners, and some of them were confirmed to be formed in cellular nucleotide pools after X-ray irradiation. We found that various modified nucleosides are generated in free purine nucleosides, DNA and RNA by X-ray irradiation in a dose dependent manner and free purine nucleosides are most susceptible to modification by X-ray irradiation among those examined. In addition, we indicated that short-term memory is affected by cranial irradiation in mice.

Free Research Field

放射線影響学