2014 Fiscal Year Final Research Report
Elucidation of the molecular mechanism of chromosome translocation formation/suppression after exposure to ionizing radiation
| Project/Area Number |
24710063
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Nagasaki University (2013-2014)
Central Research Institute of Electric Power Industry (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 染色体転座 / DNA二本鎖切断 / フォーカス / 会合 / Ku80 / DNA-PKcs / ATM / 53BP1 |
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| Outline of Final Research Achievements |
Chromosome translocation is a clinically relevant chromosome aberration since it can cause leukemia, solid cancer, or other diseases, yet the molecular mechanism for translocation formation/suppression is unknown. In the present study, we identified factors affecting interaction between multiple DNA double-strand breaks (DSBs), that is prerequisite for translocation formation. We visualized DSBs by “foci” of a DNA repair factor accumulating at DSB sites, and examined frequency of DSB interaction. We identify Ku80, DNA-PKcs, and ATM as factors suppressing DSB interaction, and we also find that 53BP1 promotes DSB interaction and thereby facilitates translocation formation.
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| Free Research Field |
放射線生物学
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