Investigation of endocrine-disrupting chemical effect for developmental process of organism using novel critical-window exposure method.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24710072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Ariake National College of Technology
• Principal Investigator: YAMAGUCHI akemi 有明工業高等専門学校, その他部局等, 技術専門職員 (90399262)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 生物・生体工学 / 電力工学 / 発生・分化 / マイクロアレイ

Outline of Final Research Achievements

We developed novel material incorporation technic into medaka fertilized eggs using high voltage pulse systems. We assessed the effects of estradiol-17β (E2), bisphenol A (BPA) or BPA relative compounds such as bisphenol S (BPS) for medaka eggs by the incorporation technic and observed by microscope. It was observed that embryonic malformation and abnormal formation of bone, hart, blood vessel and hemocyte chemicals dose-dependently. In addition, we carried out DNA microarray analysis of medaka eggs treated with E2, BPA, BPA relative compounds, and detected variation of each chemicals specific genes expressions. It is suggested that these genes relation to morphological abnormality induced by each chemicals treatment.

Free Research Field

環境科学