2013 Fiscal Year Final Research Report
Bioethanol production from bamboo culms using selective lignin-degrading basidiomycetes
| Project/Area Number |
24710085
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Miyazaki Prefectural Wood Utilization Research Center |
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Principal Investigator |
SUHARA Hiroto 宮崎県木材利用技術センター, その他部局等, 研究員 (90423540)
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| Co-Investigator(Renkei-kenkyūsha) |
KAMEI Ichiro 宮崎大学, 農学部, 准教授 (90526526)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | バイオエタノール / 担子菌類 / タケ / イネ科植物 / 選択的脱リグニン |
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| Research Abstract |
Pretreatment for bioethanol production from bamboo culms and rice straw was examined using basidiomycetous fungus Punctularia sp. TUFC20056, which has selective lignin removal ability of bamboo. A class II peroxidase gene was partially obtained from this fungus, and it codes manganese peroxidase (MnP). Optimal cultural conditions for producing lignin-degrading enzymes were as follows; laccase was 21- 25 C, 3-4 weeks or more, on solid culture condition; MnP was 21- 25 C, 3 weeks, in liquid culture.
Fermentable sugar yields from Punctularia sp.-treated bamboo (10%) and rice straw (46%) were higher than that of famous lignin degrader Phanerochaete sordida (2.4 and 8.9%, respectively) and Ceriporiopsis subvermispora. (6.1 and 9.7%, respectively). Punctularia sp. cannot ferment cellulose directly. But, this fungus can produce ethanol by adding cellulase under an anerobic condition, and the yields from pretreated-bamboo and -rice straw were 25.2 and 37.5%, respectively.
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