2013 Fiscal Year Final Research Report
Fabrication of cell-based bio-microdevice by bottom-up approach
| Project/Area Number |
24710142
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Yokohama City University (2013)
The University of Tokyo (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | バイオマイクロデバイス / 自己組織化 / 膵島 / 微小流路 / ハイドロゲルビーズ |
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| Research Abstract |
In this study, we aimed to establish the methods for fabrication of three-dimensional "bio-microdevice" that is mainly comprised of single cells. In addition, we tried to reveal the mechanisms of self-organization occurring in the process of the fabrication of bio-microdevices.
We found that aggregation and cultivation of hepatic cells and hydrogel beads resulted in the formation of network-like microvessels in the hepatic spheroids.We also established a method for fabrication of the pseudo-islet using pancreatic alpha and beta cells. The pseudo-islet acquired not only higher insulin production rate in a specific alpha/beta ratio but also the real islet-like structure. Moreover, it was revealed that sugar chains were involved in the cell-rearrangement.
These results indicate that development of the processes and understanding of the mechanisms to fabricate "bio-microdevice" lead to innovative product like the pseudo-islet.
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[Book] Three-dimensional culture of fetal mouse, rat and porcine hepatocytes. in"Human Fetal Tissue Transplantation"(etd. by N. Bhattacharya and P. Stubblefield)2013
Author(s)
Sakai, Y., Jiang, J., Hanada, S., Huang, H., Katsuda, T., Kojima, N., Teratani, T., Ochiya, T.
Total Pages
47-63
Publisher
Springer-Verlag UK
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[Remarks] 受賞① 小島伸彦, 第4回BEANS総合研究会, 最優秀発表賞, MEMSと肝臓, 2012年5月22日
