2013 Fiscal Year Final Research Report
Multilayered Cell Assembling Technology Using Dielectrophoresis and Construction of Multilayered Skin Tissue Array
| Project/Area Number |
24710145
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Keio University |
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Principal Investigator |
MIYATA Shogo 慶應義塾大学, 理工学部, 准教授 (70376515)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 多階層組織 / 真皮 / 表皮 / 誘電泳動 / 細胞チップ |
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| Research Abstract |
The objective of this study was to reconstruct multilayered structure of micro organization using dielectrophoresis (DEP). DEP is a phenomenon in which a force is generated on a cell or particle when it is subjected to a non-uniform electric field. In this study, alginate-collagen micro-beads were developed for dielectrophoresis experiments and cell adhesive scaffold. Dermal and epi-dermal skin cells were assembled by DEP force to cover the beads and to construct multilayered structure. After the cell assembly, the cell-accumulated beads were cultured in vitro to reconstruct maultilayered skin tissue. Finally, the cell viability and proliferation of the cultured constructs were maintained during 7 days culture.
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