2013 Fiscal Year Final Research Report
Molecular function of Krimp in piRNA biogenesis in Drosophila
| Project/Area Number |
24710218
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATO Kaoru 東京大学, 理学(系)研究科(研究院), 助教 (20548507)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | RNAサイレンシング / piRNA / トランスポゾン / 生殖細胞 / Krimp / ショウジョウバエ / RNA干渉 / 細胞内顆粒 |
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| Research Abstract |
piRNA is a germline-specific class of small non-coding RNA, and functions by specifically interacting with PIWI proteins. In germline cells, piRNA thereby suppresses the expression of transposable elements, so piRNA is thought to protect the germline genome from such harmful element. piRNAs are thought to be produced by two distinct mechanisms, primary pathway and secondary pathway. A Drosophila PIWI protein AGO3 interacts with piRNAs produced only by the secondary pathway. In this study, I found that a Drosophila piRNA factor Krimper (Krimp) specifically interacts with AGO3 and regulates its piRNA production, indicating that Krimp is a factor regulating the secondary pathway. In addition, I found that Krimp regulates piRNAs are not loaded on AGO3 by the primary pathway.
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Research Products
(6 results)
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[Presentation] Essential roles of a Tudor domain-containing protein2014
Author(s)
佐藤薫, 岩崎由香, 渋谷あおい, Piero Carninci, 石津弘嗣, 塩見美喜子, 塩見春彦
Organizer
Krimper, in piRNA biogenesis in Drosophila ovaries 2014 Keystone Symposia Conference - A9: RNA Silencing
Place of Presentation
Sheraton Seattle Hotel, Seattle, Washington, USA
Year and Date
20140131-0205
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