2013 Fiscal Year Final Research Report
Analysis of molecular mechanism of light harvesting complex-like protein
| Project/Area Number |
24710219
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
MYOUGA Fumiyoshi 独立行政法人理化学研究所, 環境資源科学研究センター, 研究員 (10342859)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 植物ゲノム / 葉緑体 / 光合成 / 強光ストレス / シロイヌナズナ / 複合体 |
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| Research Abstract |
Arabidopsis contain a gene coding for single predicted transmembrane helix protein called OHP1 with a light-harvesting chlorophyll a/b-binding (LHC) motif. Significant amounts of OHP1 protein were present in green tissue but their level increased in response to high-light stress. In this study we found that ohp1 mutants showed pale-green phenotype on agar plates. Leaf chlorophyll fluorescence analysis and thylakoid membrane composition indicated that ohp1 mutants had defects in PSII supercomplexes, but did not affect the accumulation of LHCs. We also generated transgenic plants expressing the tag fusion protein in ohp1 mutant that complement its pale-green phenotype. From the results of several biochemical analyses for revealing the localization of OHP1 on thylakoid membrane, the association of LHC-II with the OHP proteins has been deduced. These results suggest that OHP1 plays a fundamental role in assembly of photosystem II and is working around the LHCII SCPs as oligomers.
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Research Products
(16 results)
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[Presentation] Chloroplast function database II : a large-scale collection of homozygous mutants and their phenotype effects for nuclear-encoded chloroplast proteins2013
Author(s)
Myouga F, Akiyama K, Tomonaga Y, Kato A, Sato Y, Kobayashi M, Nagata N, Sakurai T, Schinozaki, K
Organizer
International Conference on Arabidopsis Research (ICAR 2013)
Place of Presentation
Sydney, Australia
Year and Date
20130624-28
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